A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model

Craig S. McLachlan; Jian Lin Yin; Catherine Driussi; Patricia R. Jusuf; Brett Hambly; Mark A. McGuire

doi:10.2170/jjphysiol.50.281

A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model

Craig S. McLachlan, Jian Lin Yin, Catherine Driussi, Patricia R. Jusuf, Brett Hambly, Mark A. McGuire

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.

Original language	English
Pages (from-to)	281-284
Number of pages	4
Journal	Japanese Journal of Physiology
Volume	50
Issue number	2
DOIs	https://doi.org/10.2170/jjphysiol.50.281
Publication status	Published - Apr 2000
Externally published	Yes

Keywords

Apoptosis
Head failure
PCR ladder

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10.2170/jjphysiol.50.281

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abstract = "Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.",

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AU - Yin, Jian Lin

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AU - Jusuf, Patricia R.

AU - Hambly, Brett

AU - McGuire, Mark A.

PY - 2000/4

Y1 - 2000/4

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AB - Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.

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KW - Head failure

KW - PCR ladder

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A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model

Abstract

Keywords

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