Abstract
Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.
Original language | English |
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Pages (from-to) | 281-284 |
Number of pages | 4 |
Journal | Japanese Journal of Physiology |
Volume | 50 |
Issue number | 2 |
DOIs | |
Publication status | Published - Apr 2000 |
Externally published | Yes |
Keywords
- Apoptosis
- Head failure
- PCR ladder