A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model

Craig S. McLachlan, Jian Lin Yin, Catherine Driussi, Patricia R. Jusuf, Brett Hambly, Mark A. McGuire

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2 Citations (Scopus)

Abstract

Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.

Original languageEnglish
Pages (from-to)281-284
Number of pages4
JournalJapanese Journal of Physiology
Volume50
Issue number2
DOIs
Publication statusPublished - Apr 2000
Externally publishedYes

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Keywords

  • Apoptosis
  • Head failure
  • PCR ladder

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