A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model

Craig S. McLachlan; Jian Lin Yin; Catherine Driussi; Patricia R. Jusuf; Brett Hambly; Mark A. McGuire

doi:10.2170/jjphysiol.50.281

A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model

Craig S. McLachlan, Jian Lin Yin, Catherine Driussi, Patricia R. Jusuf, Brett Hambly, Mark A. McGuire

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.

Original language	English
Pages (from-to)	281-284
Number of pages	4
Journal	Japanese Journal of Physiology
Volume	50
Issue number	2
DOIs	https://doi.org/10.2170/jjphysiol.50.281
Publication status	Published - Apr 2000
Externally published	Yes

Keywords

Apoptosis
Head failure
PCR ladder

Access to Document

10.2170/jjphysiol.50.281

Cite this

@article{e45e6cedd7834f719aa20d6203ba1c3a,

title = "A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model",

abstract = "Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.",

keywords = "Apoptosis, Head failure, PCR ladder",

author = "McLachlan, {Craig S.} and Yin, {Jian Lin} and Catherine Driussi and Jusuf, {Patricia R.} and Brett Hambly and McGuire, {Mark A.}",

year = "2000",

month = apr,

doi = "10.2170/jjphysiol.50.281",

language = "English",

volume = "50",

pages = "281--284",

journal = "Journal of Physiological Sciences",

issn = "1880-6546",

publisher = "Springer Japan",

number = "2",

}

A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model. / McLachlan, Craig S.; Yin, Jian Lin; Driussi, Catherine; Jusuf, Patricia R.; Hambly, Brett; McGuire, Mark A.

In: Japanese Journal of Physiology, Vol. 50, No. 2, 04.2000, p. 281-284.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model

AU - McLachlan, Craig S.

AU - Yin, Jian Lin

AU - Driussi, Catherine

AU - Jusuf, Patricia R.

AU - Hambly, Brett

AU - McGuire, Mark A.

PY - 2000/4

Y1 - 2000/4

N2 - Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.

AB - Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.

KW - Apoptosis

KW - Head failure

KW - PCR ladder

UR - http://www.scopus.com/inward/record.url?scp=0033934368&partnerID=8YFLogxK

U2 - 10.2170/jjphysiol.50.281

DO - 10.2170/jjphysiol.50.281

M3 - Article

C2 - 10880887

AN - SCOPUS:0033934368

VL - 50

SP - 281

EP - 284

JO - Journal of Physiological Sciences

JF - Journal of Physiological Sciences

SN - 1880-6546

IS - 2

ER -

A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this