Acetylation of VGLL4 Regulates Hippo-YAP Signaling and Postnatal Cardiac Growth

Zhiqiang Lin, Haidong Guo, Yuan Cao, Sylvia Zohrabian, Pingzhu Zhou, Qing Ma, Nathan VanDusen, Yuxuan Guo, Jin Zhang, Sean M. Stevens, Feng Liang, Qimin Quan, Pim R. van Gorp, Amy Li, Cristobal dos Remedios, Aibin He, Vassilios J. Bezzerides, William T. Pu

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

Binding of the transcriptional co-activator YAP with the transcription factor TEAD stimulates growth of the heart and other organs. YAP overexpression potently stimulates fetal cardiomyocyte (CM) proliferation, but YAP's mitogenic potency declines postnatally. While investigating factors that limit YAP's postnatal mitogenic activity, we found that the CM-enriched TEAD1 binding protein VGLL4 inhibits CM proliferation by inhibiting TEAD1-YAP interaction and by targeting TEAD1 for degradation. Importantly, VGLL4 acetylation at lysine 225 negatively regulated its binding to TEAD1. This developmentally regulated acetylation event critically governs postnatal heart growth, since overexpression of an acetylation-refractory VGLL4 mutant enhanced TEAD1 degradation, limited neonatal CM proliferation, and caused CM necrosis. Our study defines an acetylation-mediated, VGLL4-dependent switch that regulates TEAD stability and YAP-TEAD activity. These insights may improve targeted modulation of TEAD-YAP activity in applications from cardiac regeneration to cancer.

Original languageEnglish
Pages (from-to)466-479
Number of pages14
JournalDevelopmental Cell
Volume39
Issue number4
DOIs
Publication statusPublished - 21 Nov 2016
Externally publishedYes

Keywords

  • acetylation
  • cardiac
  • cardiomyocyte
  • degradation
  • Hippo-YAP pathway
  • necrosis
  • proliferation
  • TEAD1
  • VGLL4

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