Effects of high-fat diet on eHSP72 and extra-to-intracellular HSP70 levels in mice submitted to exercise under exposure to fine particulate matter

Iberê Machado Kostrycki, Guilherme Wildner, Yohanna Hannah Donato, Analú Bender dos Santos, Lílian Corrêa Costa Beber, Matias Nunes Frizzo, Mirna Stela Ludwig, Kevin Noel Keane, Vinicius Cruzat, Cláudia Ramos Rhoden, Thiago Gomes Heck

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Abstract

Obesity, air pollution, and exercise induce alterations in the heat shock response (HSR), in both intracellular 70 kDa heat shock proteins (iHSP70) and the plasmatic extracellular form (eHSP72). Extra-to-intracellular HSP70 ratio (H-index = eHSP70/ iHSP70 ratio) represents a candidate biomarker of subclinical health status. This study investigated the effects of moderate- and high-intensity exercise in the HSR and oxidative stress parameters, in obese mice exposed to fine particulate matter (PM 2.5 ). Thirty-day-old male isogenic B6 129 F 2 /J mice were maintained for 16 weeks on standard chow or high-fat diet (HFD). Then, mice were exposed to either saline or 50 μg of PM 2.5 by intranasal instillation and subsequently maintained at rest or subjected to moderate- or high-intensity swimming exercise. HFD mice exhibited high adiposity and glucose intolerance at week 16th. HFD mice submitted to moderate- or high-intensity exercise were not able to complete the exercise session and showed lower levels of eHSP70 and H-index, when compared to controls. PM 2.5 exposure modified the glycaemic response to exercise and modified hematological responses in HFD mice. Our study suggests that obesity is a critical health condition for exercise prescription under PM 2.5 exposure.

Original languageEnglish
Article number4858740
JournalJournal of Diabetes Research
Volume2019
DOIs
Publication statusPublished - 1 Jan 2019

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Kostrycki, I. M., Wildner, G., Donato, Y. H., dos Santos, A. B., Beber, L. C. C., Frizzo, M. N., Ludwig, M. S., Keane, K. N., Cruzat, V., Rhoden, C. R., & Heck, T. G. (2019). Effects of high-fat diet on eHSP72 and extra-to-intracellular HSP70 levels in mice submitted to exercise under exposure to fine particulate matter. Journal of Diabetes Research, 2019, [4858740]. https://doi.org/10.1155/2019/4858740