@inbook{01cd53fb9b0345588752e0938b754145,
title = "Maldi-tof ms: A rapid methodology for identifying and subtyping listeria monocytogenes",
abstract = "Listeria monocytogenes is a major food-borne pathogen and causative agent of a fatal disease, listeriosis. Stringent regulatory guidelines and zero tolerance policy toward this bacterium necessitate rapid, accurate, and reliable methods of identification and subtyping. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) has recently become a method of choice for routine identification of pathogens in clinical settings and has largely replaced biochemical assays. Identification relies on well-curated databases such as SARAMIS. Extensive use of SARAMIS to generate consensus mass spectra, in conjunction with statistical analysis, such as partial least square-discriminant analysis and hierarchical cluster analysis, is useful in subtyping bacteria. While MALDI-ToF MS has been extensively used for pathogen detection, its application in bacterial subtyping has been limited. The protocol describes a MALDI-ToF MS workflow as a single tool for simultaneous identification and subtyping of L. monocytogenes directly from solid culture medium.",
keywords = "Axima Performance, Identification, Listeria monocytogenes, MALDI-ToF MS, MetaboAnalyst, SARAMIS, SIMCA, Subtyping",
author = "Jadhav, {Snehal R.} and Shah, {Rohan M.} and Palombo, {Enzo A.}",
note = "Funding Information: The authors would like to thank the Australian Meat Processor Corporation and the Victorian Department of Health for providing funding support. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-0982-8_2",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "17--29",
booktitle = "Methods in Molecular Biology",
}