Nitric oxide and redox state measurements in pancreatic beta cells

Rodrigo Carlessi, Vinicius Cruzat, Younan Chen, Philip Newsholme

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The role of oxidative stress in the pathogenesis of type 2 diabetes (T2D), especially pancreatic β-cell dysfunction and death, has become apparent in the last two decades. Peroxidase- and catalase-based antioxidant mechanisms are particularly weak in β-cells and can be easily overwhelmed by excessive production of reactive oxygen and nitrogen species in the course of pathological processes. Recent research has attempted to define in detail the mechanistic aspects of oxidative stress-induced β-cell dysfunction. Here, we describe the procedures for the measurement of various parameters important to assess oxidative stress in pancreatic β-cells. Detailed protocols for determination of nitric oxide (NO) production, the glutathione redox status, and general oxidative status in β-cells are presented in this chapter.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages241-253
Number of pages13
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in Molecular Biology
Volume2076
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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Keywords

  • Glutathione
  • Nitric oxide
  • Oxidative stress
  • Type 2 diabetes mellitus (T2DM)
  • β-cells

Cite this

Carlessi, R., Cruzat, V., Chen, Y., & Newsholme, P. (2020). Nitric oxide and redox state measurements in pancreatic beta cells. In Methods in Molecular Biology (pp. 241-253). (Methods in Molecular Biology; Vol. 2076). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9882-1_14