Screening for Differential Gene Expression during the Development of Form-Deprivation Myopia in the Chicken

Ian Morgan, Robert Kucharski, Nispa Krongkaew, Sally I. Firth, Pam Megaw, Ryszard Maleszka

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Purpose. To use the technique of differential gene display to analyze changes in gene expression that occur during the development of and recovery from form-deprivation myopia. Methods. The differential display-reverse transcriptase-polymerase chain reaction technique was used to detect cDNAs that are differentially expressed after 24 h (including 12 h in the light) after fitting with a diffuser to induce form-deprivation myopia. Messenger RNA levels were determined by quantitative Northern blotting in retinas after 11 days of form deprivation or in retinas where the diffusers had been removed the previous day. Results. Twenty-six differentially expressed genes were processed in our initial screen. Two of these, αB-crystallin and retinoic acid receptor-α, were studied further. Levels of αB-crystallin mRNA were increased on day 11 in retinas from form-deprived eyes relative to eyes of control chickens and were reduced to below those levels within 6 to 12 h after removal of the diffusers. Levels of retinoic acid receptor-α mRNA showed similar changes, except that after removal of the diffusers, the levels further increased. Conclusions. The technique of differential gene display can be used to detect changes in gene expression during the regulation of eye growth. The response of αB-crystallin is particularly interesting because expression increases when eye growth is high and decreases when eye growth slows.

Original languageEnglish
Pages (from-to)148-155
Number of pages8
JournalOptometry and Vision Science
Volume81
Issue number2
DOIs
Publication statusPublished - 1 Feb 2004
Externally publishedYes

Keywords

  • Crystallin
  • Gene expression
  • Myopia
  • Retina
  • Retinoic acid

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